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KMID : 1206219990060030063
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Journal of Korean Academy of Physical Therapy Science
1999 Volume.6 No. 3 p.63 ~ p.82
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Effects of ultraviolet light B irradiation on nitric oxide activity in the sprague-dawley rat in vivo (I)
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Min Kyung-Ok
Kim Jung-Hwan
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Abstract
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Recent studies have revealed that Nitric oxide(NO) was one of the demonstration for the physiological regulator, endothelial derived relaxing factor(EDRF) and that NO was produced by ultraviolet irradiation in human. Thus, the present author have carried out a experimental study on the change of hematological, histological value of ultraviolet irradiation in sprague-dawley rats. The subjects were divided into four groups of ten rats each selected at random. There were 4 groups: 1. no irradiation control; 2. ultraviolet 75mJ/㎠ irradiation group; 3. ultraviolet 150mJ/㎠ irradiation group; 4. ultraviolet 225mJ/㎠ group. After a irradiation, hematological and histological tests were performed to observe erythrocyte, hemoglobin, hematocrit, MCH, MCHC, MCV,O₂saturation, pH,PO₂,PCO₂value and to observe histological changes. In hematological tests, erythrocyte, hemoglobin, hematocrit significantly increased in 75mJ/㎠ than control group and more 150mJ/㎠ ultraviolet irradiation group respectively. Also In blood gas tests, PO₂significantly increased in 75mJ/㎠ and more 150mJ/㎠ group than control group. Whereas PCO₂significantly decreased in 75mJ/㎠ and more 150mJ/㎠ group than control group (Duncan-Tukey test, P<0.05). In histological tests, control and 75mJ/㎠ group unchanged, but more 150mJ/㎠ group changed that it was cytolysis, cytotoxic effect, acanthosis, proliferation of keratinocyte, appearance of amorphous cell and pyknotic nucleus, production of sunburn cell. In conclusion, the present author results support the importance of the relation between NO effect and hematological, histological value by ultraviolet B irradiation.
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KEYWORD
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ultraviolet, nitric oxide(NO), hematological changes, histological changes
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